Plant Tissue Culture for the Classroom and Home
An Interactive CD with Lessons,
Lectures, Labs, and Technical Manual
Version 1.1
- "Plant Tissue Culture for the Classroom and Home: An
Interactive CD with Lessons, Lectures, Labs, and Technical Manual" was developed to
include more in-depth information and "how-to" photo layouts than could be
included in the original manual from the "Kitchen Culture Kit".
- It is organized in a series of lessons which progress from
simple media preparation, plant tissue disinfestation and culture, and transfer of the new
plants to soil.
- Lab exercises show you, in photos, how to do each procedure.
Interwoven in the lessons and labs are optional lectures which explain what tissue culture
is, what plant hormones do, methods of disinfection using different chemical
disinfectants, different paths of development and how to maintain true clones, and some
higher tech procedures like protoplast culture, anther culture, and genetic engineering.
- Specific protocols for various plant species are included,
and new ones are added to an interactive webpage as they are developed or requested by
others.
- The appendix includes a "growing glossary" which
will continue to grow via additions to the webpage, MSDS for relevant chemicals, FAQs, and
a technical manual, "Manual of Plant Tissue Culture Methods" (in Adobe Acrobat
pdf format) which includes various media formulations, methods to make them "from
scratch", detailed protocols and lab exercises suitable for classroom and laboratory
use.
- The protected webpage, which can be accessed by CD owners,
will enable the CD to be dynamic with new information being continually added.
- A restricted listserv will enable CD owners to communicate
easily with each other to exchange ideas, and, in the tradition of Kitchen Culture Kits,
Inc., there will continue to be interactive support via phone, fax, or email.
- The CD was written using the ISO 9660 File System Option to
enable reading the CD on different platforms including DOS, Macintosh, OS/2, Windows and
UNIX.
- Check out the Introduction and Table of Contents.
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