Materials Needed
- Culture plates made earlier.
- Spore print made earlier.
- Marking pen.
- Knife.
- Propane torch.
- Fire Extinguisher
Procedure
- Be sure to work in a clean, low traffic area to help prevent
contamination.
- Light the torch and keep it at a low flame. Extreme care must be taken when working with this. Follow
manufacturer's recommendations on safety.
- Pass the knife over the flame until the tip becomes red-hot.
- Allow the tip to cool, this may take a minute or two.
- Gently scrape one small area of the spore print, try to just pick up
the spores of the mushroom on the knife and leave the paper fiber.
- Carefully open one of the culture plates, just exposing enough of the
inside of the plate to enable the knife room to smear the spores over the surface of the
media.
- Promptly close the culture plate tightly to avoid contamination.
- Label the plate with the mushroom type and the date.
- Clean any excess materials off of the knife.
- Repeat this procedure until you have the desired number of plates.
- Place the plates in a warm dark location where they will not be
disturbed
- In several days, a white, stringy growth should begin to appear on
the surface of the media. This material is mushroom mycelium.
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