Workshop: All Day Session
Plant Tissue Culture in the Classroom and Home
for the Serious Teacher, Nurseryman, Hobbyist or
Gardner
(includes lunch and a Kitchen Culture Kit)
Thursday - Friday (To be Announced) 9 a.m. to 5 p.m. - Room S111
Email us for specific dates: carolstiff@kitchenculturekit.com
Angelina College, Lufkin, TX
- Plant
tissue culture involves the sterile growth of plants in bottles for the purpose of mass
production.
Through the use of plant hormones and
other growth regulators, small plant parts can be induced to produce hundreds of small
"plantlets" which can be further developed and grown in greenhouses or as house
plants.
Using a microwave oven or a pressure
cooker, supplies found in your kitchen, plus the contents of the newly developed
"Kitchen Culture Kit©, you can mass propagate hundreds of your favorite
plants in your kitchen or classroom.
In this class you will:
make your own media
disinfect and culture plant leaves,
axillary buds and seeds
transfer older cultures to fresh media or
to soil
culture a plant of your choice (time
permitting - contact the instructor prior to class: kck@turbonet.com )
receive lunch, a "Kitchen Culture
Kit" with manual, supplies, and chemicals for media preparation, and 8 take-home
plant cultures
All materials will be provided
Tour a tissue culture lab/nursery (day 2)
|
Instructor: Carol Stiff is a Ph.D. plant tissue culture specialist and president
of Kitchen Culture Kits, Inc., Lufkin, TX
www.kitchenculturekit.com
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Tentative Schedule - Day 1
|
| 9:00 a.m. |
Introductions and overview |
| 9:15 a.m. |
Slide presentation on plant tissue culture
concepts, use in industry, and using home tissue culture methods |
| 10:15 a.m. |
Break: (no beverages allowed at culture
table once hands-on begins) |
| 10:30 a.m. |
Media preparation using a microwave (with a
demonstration of using a pressure cooker) |
| 12 Noon |
Organized lunch with whole class - will serve
as a group discussion |
| 1:15 p.m. |
Demonstration/Hands-on:
Disinfection and culture of African
violet leaves and axillary buds
Transfer of established cultures to
fresh media
Disinfestation and culture of seeds
including an orchid seed demonstration
Disinfestation and culture of your
plant of choice (need to contact instructor ahead of time)
|
| 3:30 p.m. |
Break |
| 4:00 p.m. |
Options:
- Demonstration of making a homemade filter
- Dissection of difficult to locate meristems
- Using biocides (PPM, NaDCC)
- Finish cultures and pack up cultures and supplies
- Student's choice within the capabilities of the instructor
Discuss problems, alternative protocols, internet
connection and webpage resources, finish cultures and pack up cultures and supplies |
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